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Enhanced poly ADP-ribosylation in human leukemia lymphocytes and ovarian cancers.

Posted on 05/07/2010
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Freshly isolated human peripheral blood lymphocytes from leukemia (AML, ALL, CML) subjects, showed a 2.5-3.5-fold increase in the poly ADPR transferase (poly ADPRT) activity whereas ovarian cancers showed a 2-fold increase.

This was accompanied by a drop in NAD levels of 45%-63% in leukemia cells and 40% in ovarian cancers. Tumour promoters phorbol-12-myristate-13-acetate (PMA) and mezerein produced an increase in poly ADPRT activity in both normal and CML lymphocytes, but the increase was more marked in the case of normals. This was accompanied by a drop in NAD levels.

The results presented show a marked increase in poly ADP-ribosylation in malignant cells but normal lymphocytes showed a greater response to tumour promoters as compared to CML lymphocytes.

About the Authors

Singh N. (1991) Enhanced poly ADP-ribosylation in human leukemia lymphocytes and ovarian cancers. Cancer Lett. 1991 Jun 14;58(1-2):131-5. Department of Biochemistry, All India Institute of Medical Sciences, New Delhi.

Comments

Dr_Abram_Hoffer

Posted on 05/16/2010 01:46 pm

This new work with NAD Therapy is very exciting and I think is right on target. It is indeed an energy-metabolic-deficiency (EMD) because in the absence of this coenzyme cycle almost all the reactions in the body run down... I congratulate Theo Verwey and his colleagues for this remarkable advance in using this concept and in using a simple test, the ratio of pyruvate to lactate as a diagnostic measure, to indicate the dose, duration of treatment etc.